Star activity of restriction enzymes

Title:

Star activity of restriction enzymes

Description:

This site has interesting information on Restriction Enzyme protocols. An Excerpt from the page I'm recommending:

"There are some restriction enzymes which cleave partially different base sequence than recognition sequence as a result of reduced specificity when used in a large excess to substrate DNA. This phenomenon is called Star Activity. The list of enzymes for which Star Activity is noticed are shown below and the conditions to accelerate its appearance, and the base sequences cleaved by Star Activity for which such reports are available."

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