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Contamination Problems - PCR

I'm soooo frustrated over my PCR work. I keep getting false positives even when I set my reactions only with water. This must be due to contamination.

I used filter-tips and proper gloves, I washed the pipettes thoroughly etc.

Anyone have any tips on what could be wrong?


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bioguy's picture

Comment #153: Try irradiating the thermocycler with UV

Submitted by bioguy on June 25, 2005 - 12:49am
In response to the main content

done this yet?

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